Berkman singled to lead off the eighth inning, but asked to be removed from the game when he felt tightness in his calf. "When I hit the single, I could tell that if I had to score from first on a double, I wasn't going to be the guy to do that the way I felt," Berkman said. "We'll see how I feel in the morning."
Rafael Furcal pinch-ran for Berkman in the eighth.
It was a chilly night at Great American Ball Park, and Berkman said the cool conditions probably contributed to the injury. Berkman, who also has been nursing a sore hand, went 2-for-3 on Tuesday with a walk, triple and single in the Cardinals' 3-1 victory over the Reds.
"He was a little tight in his calf," said Cardinals manager Mike Matheny. "On these kind of nights you do what you can to keep guys warm."