Holliday has some tightness in his left quadriceps and believes he will be able to play in Friday's game in Kansas City. Berkman will likely be out at least a few days due to a sprained right wrist.
"I haven't run yet, but I think we're good," Holliday said on Thursday afternoon.
It's possible that Holliday will be used as the Cards' designated hitter on Friday, their first game of Interleague Play for the 2011 season. Berkman is not likely to play for longer than that, though he is not headed for the disabled list.
"It's not torn," Berkman said. "It's not broken. It's like spraining your ankle -- as soon as it feels better to run, you can run. It's going to be the same thing."
Holliday was removed from Wednesday's game in the third inning after feeling discomfort running out a ball in the second. Berkman came out two innings later, the result of an awkward tumble when he caught a line drive.
In a bit of good news for the Redbirds, though, center fielder Colby Rasmus returned to the lineup from injury on Thursday. Rasmus had not started since Saturday, the result of an abdominal strain. He went 1-for-3 and scored a run in the 4-2 victory.
Allen Craig started in left field, going 3-for-4 with a solo homer, and Jon Jay in right for St. Louis. Jay went 0-for-4 and made a sliding catch in the seventh on a drive by Brett Wallace.